Marmara Üniversitesi Açık Arşiv Sistemi

Sequence specific recognition of ssDNA by fluorophore 3-hydroxyflavone

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dc.contributor.author Bostan Sennaroglu, Muge
dc.contributor.author Eroǧlu, Mehmet S.
dc.date.accessioned 2016-01-27T12:53:49Z
dc.date.available 2016-01-27T12:53:49Z
dc.date.issued 2015
dc.identifier.issn 10111344
dc.identifier.uri https://goo.gl/xYU2rj
dc.identifier.uri http://hdl.handle.net/11424/4193
dc.description.abstract A fully water soluble 3-hydroxyflavone (3HF) derivative, N-(3-hydroxy-4′-flavonyl)-N,N,N-trimethylammonium sulfate (3HFNMe3) was synthesized. Investigation of its emissions at varying wavelengths revealed that it had three emission bands of normal (N∗), anionic (A∗) and tautomeric (T∗), in ultrapure water. Recognition of single-stranded ten ssDNA chains, having different nucleotide sequences was studied, using the ratiometric change of the intensities of the two bands (A∗/T∗), depending upon the varying environment of the 3HFNMe3 with different ssDNA chains. Addition of the ssDNA chains to the 3HFNMe3 solution caused gradual quenching of the A∗ band and had almost no effect on the T∗ band. As the ratios of the two bands (A∗/T∗) vs increasing amount of the ssDNAs generated characteristic curves for each ssDNA chain, it became possible to identify the chains with their characteristic curves. © 2015 Elsevier B.V. en_US
dc.language.iso eng en_US
dc.relation.isversionof 10.1016/j.jphotobiol.2015.10.019 en_US
dc.rights info:eu-repo/semantics/restrictedAccess en_US
dc.subject 3-Hydroxyflavone; DNA; Fluorophore; Specific recognition; Spectroscopic methods en_US
dc.title Sequence specific recognition of ssDNA by fluorophore 3-hydroxyflavone en_US
dc.type article en_US
dc.contributor.authorID TR126588 en_US
dc.contributor.authorID TR38807 en_US
dc.relation.journal Journal of Photochemistry and Photobiology B: Biology en_US
dc.contributor.department Marmara University, Department of Chemical Engineering en_US
dc.identifier.volume 1153 en_US
dc.identifier.startpage 391 en_US
dc.identifier.endpage 396 en_US


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