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dc.contributor.authorAkkiprik, Mustafa
dc.contributor.authorPeker, İrem
dc.contributor.authorÖzmen, Tolga
dc.contributor.authorAmuran, Gökçe Güllü
dc.contributor.authorGüllüoǧlü, Bahadır Mahmut
dc.contributor.authorKaya, Handan
dc.contributor.authorÖzer, Ayşe
dc.date.accessioned2016-03-09T19:14:19Z
dc.date.available2016-03-09T19:14:19Z
dc.date.issued2015
dc.identifier.issn20734425
dc.identifier.urihttp://tinyurl.com/he2qfnc
dc.identifier.urihttp://hdl.handle.net/11424/4261
dc.description.abstractIGFBP5 is an important regulatory protein in breast cancer progression. We tried to identify differentially expressed genes (DEGs) between breast tumor tissues with IGFBP5 overexpression and their adjacent normal tissues. In this study, thirty-eight breast cancer and adjacent normal breast tissue samples were used to determine IGFBP5 expression by qPCR. cDNA microarrays were applied to the highest IGFBP5 overexpressed tumor samples compared to their adjacent normal breast tissue. Microarray analysis revealed that a total of 186 genes were differentially expressed in breast cancer compared with normal breast tissues. Of the 186 genes, 169 genes were downregulated and 17 genes were upregulated in the tumor samples. KEGG pathway analyses showed that protein digestion and absorption, focal adhesion, salivary secretion, drug metabolism-cytochrome P450, and phenylalanine metabolism pathways are involved. Among these DEGs, the prominent top two genes (MMP11 and COL1A1) which potentially correlated with IGFBP5 were selected for validation using real time RT-qPCR. Only COL1A1 expression showed a consistent upregulation with IGFBP5 expression and COL1A1 and MMP11 were significantly positively correlated. We concluded that the discovery of coordinately expressed genes related with IGFBP5 might contribute to understanding of the molecular mechanism of the function of IGFBP5 in breast cancer. Further functional studies on DEGs and association with IGFBP5 may identify novel biomarkers for clinical applications in breast cancer. © 2015 by the authors; licensee MDPI, Basel, Switzerland.en_US
dc.language.isoengen_US
dc.relation.isversionof10.3390/genes6041201en_US
dc.rightsinfo:eu-repo/semantics/restrictedAccessen_US
dc.subjectBreast cancer; Gene expression profiling; IGFBP5; Microarray; Pathway analysisen_US
dc.titleIdentification of differentially expressed IGFBP5-related genes in breast cancer tumor tissues using cDNA microarray experimentsen_US
dc.typearticleen_US
dc.contributor.authorIDTR38330en_US
dc.contributor.authorIDTR172842en_US
dc.contributor.authorIDTR227114en_US
dc.contributor.authorIDTR3127en_US
dc.contributor.authorIDTR11475en_US
dc.relation.journalGenesen_US
dc.contributor.departmentDepartment of Medical Biology, School of Medicine, Marmara Universityen_US
dc.identifier.volume6en_US
dc.identifier.issue4en_US
dc.identifier.startpage1201en_US
dc.identifier.endpage1214en_US


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