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Identification of differentially expressed IGFBP5-related genes in breast cancer tumor tissues using cDNA microarray experiments

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dc.contributor.author Akkiprik, Mustafa
dc.contributor.author Peker, İrem
dc.contributor.author Özmen, Tolga
dc.contributor.author Amuran, Gökçe Güllü
dc.contributor.author Güllüoǧlü, Bahadır Mahmut
dc.contributor.author Kaya, Handan
dc.contributor.author Özer, Ayşe
dc.date.accessioned 2016-03-09T19:14:19Z
dc.date.available 2016-03-09T19:14:19Z
dc.date.issued 2015
dc.identifier.issn 20734425
dc.identifier.uri http://tinyurl.com/he2qfnc
dc.identifier.uri http://hdl.handle.net/11424/4261
dc.description.abstract IGFBP5 is an important regulatory protein in breast cancer progression. We tried to identify differentially expressed genes (DEGs) between breast tumor tissues with IGFBP5 overexpression and their adjacent normal tissues. In this study, thirty-eight breast cancer and adjacent normal breast tissue samples were used to determine IGFBP5 expression by qPCR. cDNA microarrays were applied to the highest IGFBP5 overexpressed tumor samples compared to their adjacent normal breast tissue. Microarray analysis revealed that a total of 186 genes were differentially expressed in breast cancer compared with normal breast tissues. Of the 186 genes, 169 genes were downregulated and 17 genes were upregulated in the tumor samples. KEGG pathway analyses showed that protein digestion and absorption, focal adhesion, salivary secretion, drug metabolism-cytochrome P450, and phenylalanine metabolism pathways are involved. Among these DEGs, the prominent top two genes (MMP11 and COL1A1) which potentially correlated with IGFBP5 were selected for validation using real time RT-qPCR. Only COL1A1 expression showed a consistent upregulation with IGFBP5 expression and COL1A1 and MMP11 were significantly positively correlated. We concluded that the discovery of coordinately expressed genes related with IGFBP5 might contribute to understanding of the molecular mechanism of the function of IGFBP5 in breast cancer. Further functional studies on DEGs and association with IGFBP5 may identify novel biomarkers for clinical applications in breast cancer. © 2015 by the authors; licensee MDPI, Basel, Switzerland. en_US
dc.language.iso eng en_US
dc.relation.isversionof 10.3390/genes6041201 en_US
dc.rights info:eu-repo/semantics/restrictedAccess en_US
dc.subject Breast cancer; Gene expression profiling; IGFBP5; Microarray; Pathway analysis en_US
dc.title Identification of differentially expressed IGFBP5-related genes in breast cancer tumor tissues using cDNA microarray experiments en_US
dc.type article en_US
dc.contributor.authorID TR38330 en_US
dc.contributor.authorID TR172842 en_US
dc.contributor.authorID TR227114 en_US
dc.contributor.authorID TR3127 en_US
dc.contributor.authorID TR11475 en_US
dc.relation.journal Genes en_US
dc.contributor.department Department of Medical Biology, School of Medicine, Marmara University en_US
dc.identifier.volume 6 en_US
dc.identifier.issue 4 en_US
dc.identifier.startpage 1201 en_US
dc.identifier.endpage 1214 en_US

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