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dc.contributor.authorŞalva, Emine
dc.contributor.authorAkbuğa, Jülide
dc.date.accessioned2018-02-21T21:01:28Z
dc.date.available2018-02-21T21:01:28Z
dc.date.issued2017
dc.identifier.urihttp://hdl.handle.net/11424/6292
dc.description.abstractThe aim of this study was to develop pGM-CSF containing chitosan/PVP hydrogel system for gene delivery. The hydrogels in semi-interpenetrating polymer network (semi-IPN) structure were synthesized by glutaraldehyde crosslinking between 2% chitosan and 2%, %4, %6 PVP. The viscosity, swelling properties and surface morphology of hydrogels were examined. These hydrogels showed highly porous structure. The porosity was increased with enhanced PVP concentration. Viscosity decreased with increasing PVP concentration of the hydrogels. The swelling percentage increased with increasing PVP concentration. In order to examine the effect of cell attachment and proliferation of hydrogels, NIH-3T3 fibroblast cell was used. The cells were able to attach on the top surface of hydrogels. However, the attachment of cells were slow at days 1 and 3. At days 7 and 14, cells looked healthy and normal and proliferated around and top of the hydrogels. With the ELISA study, the highest GM-CSF expression was obtained with pGM-CSF containing 2% chitosan+4% PVP hydrogels. MTT assay showed that chitosan/PVP hydrogels did not induce significant cytotoxic effect. As a result, these hydrogels could be served candidate for local gene delivery in tissue regeneration.en_US
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectpGM-CSF, chitosan, PVP, hydrogel, gene deliveryen_US
dc.titleThe effects to GM-CSF expression and fibroblast proliferation of pgmcsf containing chitosan/PVP hydrogelsen_US
dc.typearticleen_US
dc.contributor.authorIDTR111228en_US
dc.contributor.authorIDTR11241en_US
dc.relation.journalMarmara Pharmaceutical Journalen_US
dc.identifier.volume21en_US
dc.identifier.issue2en_US
dc.identifier.startpage228en_US
dc.identifier.endpage234en_US


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