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Development and validation of a reversed-phase HPLC method for the determination of lisinopril and gliclazide in pharmaceuticals

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dc.contributor.author Şenkardeş, Sevil
dc.contributor.author Özaydın, Tuğçe
dc.contributor.author Uğurlu, Timuçin
dc.contributor.author Küçükgüzel, Ş. Güniz
dc.date.accessioned 2018-02-22T21:12:34Z
dc.date.available 2018-02-22T21:12:34Z
dc.date.issued 2017
dc.identifier.uri http://hdl.handle.net/11424/6306
dc.description.abstract The aim of the present study was to develop and validate a High-Performance Liquid Chromatography (HPLC) method for the determination of lisinopril and gliclazide. The method was developed on Zorbax C8 analytical column (4.6x250 mm; 5μm) by isocratic elution with a flow rate of 1.0 mL/min and injection volume of 25 μl. The mobile phase composition was methanol:water (65:35 v/v, pH adjusted to 3.0 triethylamineorthophosphoric acid buffer) and the retention time was found to be 2.883 and 7.456 min for lisinopril and gliclazide, respectively. The developed method was found to be linear in the concentration range of 5-20 μg/mL for lisinopril and 15-60 μg/mL for gliclazide. The method was validated for linearity, accuracy, precision, LOD and LOQ. This developed procedure was succesfully applied conveniently for the analysis of lisinopril and gliclazide in pharmaceutical preparations. en_US
dc.language.iso eng en_US
dc.rights info:eu-repo/semantics/openAccess en_US
dc.subject Combined dosage forms, gliclazide, lisinopril, RPHPLC, method validation en_US
dc.title Development and validation of a reversed-phase HPLC method for the determination of lisinopril and gliclazide in pharmaceuticals en_US
dc.type article en_US
dc.contributor.authorID TR49974 en_US
dc.contributor.authorID TR172084 en_US
dc.relation.journal Marmara Pharmaceutical Journal en_US
dc.identifier.volume 21 en_US
dc.identifier.issue 2 en_US
dc.identifier.startpage 338 en_US
dc.identifier.endpage 344 en_US


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