Marmara Üniversitesi Açık Arşiv Sistemi

In vitro PDGF-B Gene silencing studies and ın vivo delivery of siRNA to the Rat Kidney using Chitosan/siRNA Nanoplexes

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dc.contributor.author Şalva, Emine
dc.contributor.author Özbaş Turan, Suna
dc.contributor.author Alan, Saadet
dc.contributor.author Akbuğa, Jülide
dc.date.accessioned 2018-03-06T21:14:14Z
dc.date.available 2018-03-06T21:14:14Z
dc.date.issued 2016
dc.identifier.uri http://hdl.handle.net/11424/6341
dc.description.abstract The targeting of specific genes responsible from onset and progression of kidney diseases offer a new therapeutic strategy in the field of renal gene therapy. The altered expression of platelet derived growth factor (PDGF) is an important marker of renal diseases. In this study, we investigated in vitro gene silencing efficiency of chitosan nanoplexes containing PDGF-B and PDGFR-β targeted siRNAs in the kidney cell lines including HEK-293 and MDCK and delivery to the kidney as an in vivo delivery system. As a result, PDGF-B expression was significantly inhibited by co-delivery of chitosan/siPDGF-B and siPDGFR-β nanoplexes prepared using in the different weight ratios (10/1, 20/1 and 50/1). When 20/1 and 50/1 weight ratios of chitosan nanoplexes were i.v. injected to rats, chitosan/FITC-siPDGFB nanoplexes were reached to kidney tissue at 4 h after intravenous injection. These results suggest that delivery of siRNA using chitosan nanoplexes may be effective for the therapy of kidney diseases. en_US
dc.language.iso eng en_US
dc.rights info:eu-repo/semantics/openAccess en_US
dc.subject siRNA,PDGF-B,PDGFR-β,chitosan,nanoplexes,kidney en_US
dc.title In vitro PDGF-B Gene silencing studies and ın vivo delivery of siRNA to the Rat Kidney using Chitosan/siRNA Nanoplexes en_US
dc.type article en_US
dc.contributor.authorID TR111228 en_US
dc.contributor.authorID TR246064 en_US
dc.contributor.authorID TR11241 en_US
dc.relation.journal Marmara Pharmaceutical Journal en_US
dc.identifier.volume 20 en_US
dc.identifier.issue 3 en_US
dc.identifier.startpage 263 en_US
dc.identifier.endpage 268 en_US


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