The effect of extremly low frequency magnetic fields on membrane potential of lymphocytes
Mega Tiber, Pınar
İnhan Garip, Ayşe
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Aim: Although previous studies demonstrated that extremly low frequency magnetic fields (ELF-EMF) affected biologic systems the mechanisms has not been elucidated yet. However, it is accepted that the interaction must involve the physicochemical properties of the plasma membrane. This study aimed to determine the probable change in membrane potential of mitogen induced lymphocytes exposed to ELF-EMF and correlate it with changes in proliferation of exposed cells. Methods: Lymphocytes from healthy donors were grown in RPMI medium for 72 hours, before magnetic field ( 50 Hz, 5 mT) was applied for 90 minutes. Fluorescence spectroscopy with di-4-Anepps as a probe was used to determine change in membrane potential in the the magnetic field applied and control groups. Cell count was made with cytometer using trypan blue stain. Results: Fluorescence spectroscopy studies revealed that magnetic field applied lymphocytes showed hyperpolarization with respect to control. A decrease in proliferation was observed in ELF-EMF exposed cells with respect to controls. These findings indicate that ELF-EMF affects membrane potential and cell proliferation.