Show simple item record

dc.contributor.authorKaya, Handan
dc.date.accessioned2016-02-11T14:14:01Z
dc.date.available2016-02-11T14:14:01Z
dc.date.issued2016
dc.identifier.issn09456317
dc.identifier.urihttp://tinyurl.com/hj4xbs6
dc.identifier.urihttp://hdl.handle.net/11424/4217
dc.description.abstractMicroinvasion is the smallest morphologically identifiable stage of invasion. Its presence and distinction from in situ carcinoma may have therapeutic implications, and clinical staging also requires the recognition of this phenomenon. Microinvasion is established on the basis of several morphological criteria, which may be difficult and not perfectly reproducible among pathologists. The aim of this study was to assess the consistency of diagnosing microinvasion in the breast on traditional haematoxylin and eosin (HE) stained slides and to evaluate whether immunohistochemistry (IHC) for myoepithelial markers could improve this. Digital images were generated from representative areas of 50 cases stained with HE and IHC for myoepithelial markers. Cases were specifically selected from the spectrum of in situ to microinvasive cancers. Twenty-eight dedicated breast pathologists assessed these cases at different magnifications through a web-based platform in two rounds: first HE only and after a washout period by both HE and IHC. Consistency in the recognition of microinvasion significantly improved with the use of IHC. Concordance rates increased from 0.85 to 0.96, kappa from 0.5 to 0.85, the number of cases with 100 % agreement rose from 9/50 to 25/50 with IHC and the certainty of diagnosis also increased. The use of IHC markedly improves the consistency of identifying microinvasion. This corroborates previous recommendations to use IHC for myoepithelial markers to clarify cases where uncertainty exists about the presence of microinvasion. Microinvasive carcinoma is a rare entity, and seeking a second opinion may avoid overdiagnosis. © 2016 Springer-Verlag Berlin Heidelbergen_US
dc.language.isoengen_US
dc.relation.isversionof10.1007/s00428-016-1909-xen_US
dc.rightsinfo:eu-repo/semantics/restrictedAccessen_US
dc.subjectImmunohistochemistry; In situ carcinoma; Microinvasion; Myoepithelium; Reproducibilityen_US
dc.titleConsistency in recognizing microinvasion in breast carcinomas is improved by immunohistochemistry for myoepithelial markersen_US
dc.typearticleen_US
dc.contributor.authorIDTR3127en_US
dc.relation.journalVirchows Archiven_US
dc.contributor.departmentSchool of Medicine, Department of Pathology, Marmara Universityen_US
dc.identifier.startpage1en_US
dc.identifier.endpage9en_US


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record