Show simple item record

dc.contributor.authorSayar, Nihat Alpagu
dc.contributor.authorSarıyar Akbulut, Berna
dc.date.accessioned2016-05-08T13:09:54Z
dc.date.available2016-05-08T13:09:54Z
dc.date.issued2014
dc.identifier.issn15362310
dc.identifier.urihttp://goo.gl/PuAgTS
dc.identifier.urihttp://hdl.handle.net/11424/4524
dc.description.abstractPostgenomics drug development is undergoing major transformation in the age of multi-omics studies and drug repositioning. Rather than applications solely in personalized medicine, omics science thus additionally offers a better understanding of a broader range of drug targets and drug repositioning. Berberine is an isoquinoline alkaloid found in many medicinal plants. We report here a whole genome microarray study in tandem with proteomics techniques for mining the plethora of targets that are putatively involved in the antimicrobial activity of berberine against Escherichia coli. We found DNA replication/repair and transcription to be triggered by berberine, indicating that nucleic acids, in general, are among its targets. Our combined transcriptomics and proteomics multi-omics findings underscore that, in the presence of berberine, cell wall or cell membrane transport and motility-related functions are also specifically regulated. We further report a general decline in metabolism, as seen by repression of genes in carbohydrate and amino acid metabolism, energy production, and conversion. An involvement of multidrug efflux pumps, as well as reduced membrane permeability for developing resistance against berberine in E. coli was noted. Collectively, these findings offer original and significant leads for omics-guided drug discovery and future repositioning approaches in the postgenomics era, using berberine as a multi-omics case study. © Mary Ann Liebert, Inc.en_US
dc.language.isoengen_US
dc.relation.isversionof10.1089/omi.2013.0100en_US
dc.rightsinfo:eu-repo/semantics/restrictedAccessen_US
dc.titleAssessment of berberine as a multi-target antimicrobial: A multi-omics study for drug discovery and repositioningen_US
dc.typearticleen_US
dc.contributor.authorIDTR43315en_US
dc.contributor.authorIDTR189139en_US
dc.relation.journalOMICS A Journal of Integrative Biologyen_US
dc.contributor.departmentBioengineering Department, Marmara Universityen_US
dc.identifier.volume18en_US
dc.identifier.issue1en_US
dc.identifier.startpage42en_US
dc.identifier.endpage53en_US


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record