Determination of spatial proximity between the N-terminus and cocaine binding site of the dopamine transporter by FRET
Mega Tiber, Pınar
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The dopamine transporter (DAT) mediates uptake of dopamine from the synaptic cleft and provides rapid termination of neurotransmission. It is the site of action for different drugs of abuse, including cocaine and amphetamine. Serine 7 and 12 at the N-terminus were found to be critical residues in phosphorylation. Here, we addressed spatial proximity site relationship of N-terminal extension with respect to cocaine binding in wild type and Ser7/12Asp and Ser7/12Ala mutants.